Table 1. Evaluation of trypsinolysis efficacy using an external standard of recombinant cytochrome P450 BM-3 (CYP102A1) from gram-positive Bacillus Megaterium
|
Technical replay number |
Number of identified BM3 peptides |
Sequence coverage of BM3 (%) |
||
|
Protocol 1 |
Protocol 2 |
Protocol 1 |
Protocol 2 |
|
|
1 |
83.0 (50.0%) |
76.0 (45.8%) |
86.9 |
83.2 |
|
2 |
84.0 (50.6%) |
76.0 (45.8%) |
87.4 |
84.3 |
|
3 |
81.0 (48.8%) |
76.0 (45.8%) |
86.6 |
83.6 |
|
Mean ± Standard Deviation |
82.7 ± 1.5 |
76.0 ± 0.0 |
87.0 ± 0.4 |
83.7 ± 0.5 |