Biomedical Chemistry: Research and Methods, 2018, 1(2), e00011

The Sturgeon Ovarian Liquid and the Perch Roe Extract are Able to Enhance the Recovery of the Fibroblasts after their Stress-induced Premature Senescence

M.V. Mikhailova1, N.F. Belyaeva1, N.I. Kozlova1, K.V. Zolotarev1*, A.N. Mikhailov1, S.B. Podushka2

1Institute of Biomedical Chemistry, 8119121 Pogodinskaya Str., 10 bldg. 8, Moscow, Russia,
*e-mail: fireaxe@mail.ru
2ChNIORH LLC, 10-83 Slavy prospect, St. Petersburg, 192239 Russia

Key words: fibroblasts; premature senescence; oxidative stress; fish roe; fish ovarian liquid

DOI: 10.18097/BMCRM00011

The whole version of this paper is available in Russian.

Ovarian liquid and fish roe are valuable sources of biologically active compounds. In order to study mechanisms of aging and also to search for biologically active compounds capable of inhibiting aging, we have modelled premature senescence in human embryonic fibroblasts by incubating of cells with Н2О2. Cell senescence was assessed by staining for β-galactosidase (SA-β-Gal) at pH 6.0; cell proliferation was further evaluated by the optical method. The dried ovarian liquid of the Siberian sturgeon and the extract of the perch roe were able to enhance recovery of the cells after induction of their premature senescence caused by oxidative stress. In contrast to the extract from perch roe and the extracts from fish muscle, dried ovarian fluid gave such an effect without the growth of proliferation.

Figure 1. The effect of the lyophilized Siberian sturgeon ovarian liquid upon the LECH-T cells senescence induced by Н2О2 (SA-β-Gal staining): A – cells not treated with Н2О2; B – cells incubated with the sample for 24 h after senescence induction (i.e. treatment with 50 μM Н2О2 for 1 h); C – cells after . treatment with 50 μM Н2О2 for 1 h only (control)

Figure 1. The effect of the lyophilized Siberian sturgeon ovarian liquid and the extracts of various fish species upon the senescence (A) (the percentage of the SA-β-Gal-positive cells) and the proliferation (B) of the LECH-T cells. A: for the SIPS induction, the cells were treated with 50 μM Н2О2 for 1 h and later stained for β-Gal. The result is expressed as the ratio of the proportion of stained cells after incubation with the sample to the proportion of stained cells treated with Н2О2 without further addition of the sample. B: cell proliferation was assessed at 96 h after SIPS induction using crystal violet. The result is expressed as the ratio of the optical density after incubation with the sample to the optical density of cells treated with Н2О2 without further addition of the sample (mean ± SD). The difference was considered as statistically significant of р < 0.05

ACKNOWLEDGEMENTS

This work was performed within the framework of the Program of Fundamental Scientific Research of the State Academies of Sciences of Russia for 2013 – 2020.

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