Biomedical Chemistry: Research and Methods 2021, 4(2), e00148

Determination of Cholesterol and Triglyceride Concentrations in Serum Extracellular Vesicles Using Commercial Kits

A.A. Yakovlev1,2 *, V.D. Antonov3, T.A. Druzhkova2, A.B. Guekht2, N.V. Gulyaeva1,2

1Institute of Higher Nervous Activity and Neurophysiology RAS,
5A Butlerova str., Moscow, 117485 Russia; *e-mail: al_yakovlev@ihna.ru
2Scientifc and Practical Psychoneurological Center named after Z.P. Solovy’ov DZM,
43 Donskaya str., Moscow, 115419 Russia
3Moscow Institute of Physics and Technology (State University), 9 Institutskiy per., Dolgoprudny,Moscow region, Russia

Keywords:small extracellular vesicles; blood serum; dynamic light scattering; cholesterol; triglycerides

DOI:10.18097/BMCRM00148

The whole version of this paper is available in Russian.

Exosomes and microvesicles, collectively referred to as small extracellular vesicles (sEV) are vesicles with an average size of about 100-150 nm. Currently, the role of sEV in various aspects of signaling in the body is being actively investigated; in addition, sEV can often serve as markers of various pathologies. The active study of the sEV composition is continuing. In this study we have demonstrated that in sEV it is possible to determine cholesterol and triglycerides concentration by using commercial kits designed for serum. The technique was tested on sEV from the blood of patients diagnosed with depression and on healthy volunteers. No differences were found in the concentration of cholesterol and triglycerides in mEV from the blood serum of depressed patients and the control group. The concentration of cholesterol and triglycerides in the samples is several times higher than the sensitivity threshold of the methods set by the manufacturer of the kits.
Figure 1. Biochemical parameters in the control and depression groups. A) Particle concentration according to dynamic light scattering data; B) protein concentration in sEV, mg/ml; C) the concentration of cholesterol in sEV, the dashed line indicates the detection limit, 0.023 mmol/L; D) the concentration of triglycerides in sEV, the dashed line indicates the detection limit, 0.05 mmol/L.
Figure 2. Correlation of particle concentration with A) cholesterol concentration and B) triglyceride concentration in sEV. The straight line represents best fit with linear regression, and the dotted line represents the 95% confidence interval.

FUNDING

The article was prepared in full within the state assignment of Ministry of Education and Science of the Russian Federation № АААА-А19-119071990046-9 “Neurogenetics”

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