Biomedical Chemistry: Research and Methods 2022, 5(4), e00182

Identification of Androgen Deficiency in Infertility and Reduced Ovarian Reserve Based on HPLC-MS/MS and IHLA Measurements

S.V. Kindysheva*, A.A. Gavisova, M.A. Shevtsova, L.T. Tskhovrebova, N.L., D.A. Biryukova, N.L. Starodubtseva, T.Yu. Ivanec, V.E. Frankevich

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, 4 bld. 2 Oparina str., Moscow, 117513 Russia, *e-mail: s_kindysheva@oparina4.ru.

Keywords:HPLC-MS; IHLA; androgen deficiency states

DOI:10.18097/BMCRM00182

The whole version of this paper is available in Russian.

The androgen deficiency and associated states represent is an important problem that affects the quality of women live. The most widely the androgen influence has been studied in the reproductive period in relation to polycystic ovary syndrome; however about laboratory methods to determine the impact of androgen deficiency and its clinical manifestation in the case of young women with a reduced ovarian reserve and with infertility are still actively discussed. Clinical medicine still needs generally approved markers of androgen deficiency states and its lower reference values. In this work we illustrate the perspective of measurements of steroid hormones panel to verify the diagnosis on the basis of high performance liquid chromatography with tandem mass spectrometry and immunochemical methods.
Figure 1. ROC-curves of logistic regression models developed on the base of steroid hormones concentrations measured by the IHLA when the diagnosis of androgen deficiency was made according to the questionnaire results. The numbers correspond to the following panels of hormones used to build the model:
1- DHEAS, сortisol, 17-α-OH-progesterone, androsten-3,17-dione;
2- DHEAS, cortisol, 17-α-OH-progesterone;
3- DHEAS, cortisol, 17-α-OH-progesterone, testosterone;
4- DHEAS, cortisol, androsten-3,17-dione.
Figure 2. ROC-curves of logistic regression models developed on the base of steroid hormones concentrations measured by the HPLC-MS/MS when the diagnosis of androgen deficiency was made according to the questionnaire results. The numbers correspond to the following panels of hormones used to build the model:
1- DHEAS, cortisol, androsten-3,17-dione, corticosterone;
2- cortisol, dihydrotestosterone, androsten-3,17-dione, corticosterone;
3- cortisol, 17-α-OH-Progesterone, androsten-3,17-dione, corticosterone;
4- cortisol, androsten-3,17-dione, corticosterone, 11-deoxycortisol.

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Table 1. The results of the steroid profile MS measurement for patients with androgen deficiency(AD) and control group.

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Table 2. The results of the steroid profile IHLA measurements for patients with androgen deficiency and control group.

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Table 3. The characteristics of the logistic regression models that allow us to determine the androgen deficiency presence by the steroid hormones concentrations measured by IHLA in the patient serum.

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Table 4. The parameters of the logistic regression models that allow us to determine the androgen deficiency presence by the steroid hormones concentrations measured by IHLA in the patient serum.

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Table 5. The characteristics of the logistic regression models that allow us to determine the AD presence by the steroid hormones concentrations measured by HPLC-MS/MS method in the patient serum.

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Table 6. The parameters of the logistic regression models that allow us to determine the androgen deficiency presence by the steroid hormones concentrations measured by HPLC-MS/MS in the patient serum.

FUNDING

This work was performed within state assignment «Solving the problem of infertility in modern conditions with the aid of a clinical diagnostic model of infertile marriage development and innovate technologies in assisted reproduction programs» No.121040600410-7.

Supplementary materials are available at http://dx.doi.org/10.18097/BMCRM00182

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