Biomedical Chemistry: Research and Methods 2024, 7(1), e00214

Changes in the Metabolism of Fucose-Containing Biopolymers in the Tissues of the Gastrointestinal Tract of Experimental Animals under Immobilization Stress

A.V. Oksuzyan*, E. G. Butolin, A. A. Bryzgalina

Izhevsk State Medical Academy of the Ministry of Health of Russia, 426034, Izhevsk, Kommunarov str., 281;
*e-mail: artur30st@mail.ru

Keywords:immobilization stress; fucose-containing biopolymers; liver, gastric, small intestine tissues

DOI:10.18097/BMCRM00214

The whole version of this paper is available in Russian.

The article discusses the features of the exchange of fucose-containing biopolymers in blood serum and tissues of the gastrointestinal tract under an experimental stress model. Fucosoglycoprotein metabolism was evaluated in dynamics on the 21st, 35th, 60th, 90th days of observation by the level of total fucose and fucosidase activity. In the blood serum and in the homogenates of the liver, stomach and small intestine of immobilized animals, there is a multidirectional character of changes in the level of fucose and fucosidase activity, which manifests itself in a decrease in the amount of total fucose in the serum and liver of laboratory rodents against the background of low activity of the enzyme that destroys fucoglycoproteins. At the same time, the same changes were observed in the tissues of the stomach and small intestine, which may indicate a change in metabolism towards anabolism, except for the 35th day of the experiment, where the growth of fucose and fucosidase was visualized. These changes correlate with an increase in the concentration of corticosterone in the blood serum of rats, which is indicative of the mechanism of adaptive protection in the organs of the gastrointestinal tract.
Figure 1. Changes in the content of corticosterone, total fucose (OF) and fucosidase activity (FA) in the serum and liver of immobilized rats (reliability of differences between experience and control:* – p <0.05).

CLOSE
Table 1. Parametrs of the metabolism of fucose-containing biopolymers gastric and small intestine tissues of immobilized rats (Me [Q1;Q3]).

FUNDING

The work had no external sources of funding.

REFERENCES

  1. Khnychenko L. K., Sapronov N. S. (2003) Stress and its role in the development of pathological processes. Reviews of Clinical Pharmacology and Drug Therapy, 2(3), 2-15.
  2. Semenov V., Volkov A. (2016) The influence of stress factors of various forces on the neurohumoral and immune systems of the animal body. Farm Animal Veterinary Science, 12, 35-39.
  3. Oksuzyan A.V., Butolin E.G., Ivanov G.V. (2022) Changes in the content of fucoglycoproteins in the mucous membranes and walls of the stomach and small intestine of alcoholized rats under immobilization stress. Medical academic journal, 22(3), 37-48. DOI
  4. Volkhina I. V., Naumova N. G. (2012) Тhе comparative analysis of changes of the exchange indices for biopolymers of the connective tissue in the stomach wall at immobilisation at rats with different reaction to stress. Bulletin of the Udmurt University. The series "Biology. Earth Sciences", 1, 55-58.
  5. Oksuzyan, A.V., Nurmieva D. I., Subbotina E. S. (2022) Changes in the parameters of sialoglycoproteins in the tissues of the small intestine of alcoholized rats under conditions of immobilization. Modern Science, 4(1), 19-22.
  6. Cherenkov D. A., Rybakov Yu. A., Sanina T. V., Shkarin N. Yu., Skladnev D. A., Korneeva O. S. (2010) Fucose: biological role, obtaining and application. Biotechnology in Russia , 6, 63-71.
  7. Kononova S. V. (2017) How fucose of blood group glycotopes programs the human intestinal microbiota. Biochemistry, 82(9), 1259-1277. DOI
  8. Sharaev P. N., Kildiyarova R. R., Strelkov N. S., Butolin E.G., Ozhegov A.M. (2009) Connective tissue in childhood. State Budgetary Educational Institution of Higher Professional Education "Izhevsk State Medical Academy" of the Ministry of Health of the Russian Federation, 144 p. ISBN: 978-5-91385-018-8
  9. Protasova S. V., Butolin E. G., Oksuzyan A.V. (2010) Metabolism of carbohydrate-containing biopolymers in the liver and gastric mucosa in experimental diabetes in rats with different resistance to stress. Diabetes Mellitus, 1, 10-12.
  10. Oksuzyan, A., Maier, H., McGue, M., Vaupel, J. W., Christensen, K. (2010). Sex differences in the level and rate of change of physical function and grip strength in the Danish 1905-cohort study. Journal of Aging and Health, 22(5), 589-610. DOI
  11. Jacobsen, R., Oksuzyan, A., Engberg, H., Jeune, B., Vaupel, J. W., Christensen, K. (2008). Sex differential in mortality trends of old-aged Danes: a nationwide study of age, period and cohort effects. European journal of epidemiology, 23, 723-730. DOI