Biomedical Chemistry: Research and Methods 2024, 7(3), e00232

Cytochromes P450 as Tools for Electroenzymatic Synthesis

P.I. Koroleva*, A.A. Gilep, V.V. Shumyantseva

Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia; *e-mail: polinakoroleva1996@gmail.com

Keywords: cytochrome P450; electrocatalysis

DOI:10.18097/BMCRM00232

The whole version of this paper is available in Russian.

The electrocatalytic properties of cytochrome P450 2C9 and the cytochrome P450 2C9/FAD and cytochrome P450 2C9/FMN complexes have been studied using a two-electrode system. The system consisted of an enzymatic catalyst electrode modified by the membrane-like compound didodecyldimethylammonium bromide (SPE/DDAB) and a measuring electrode, modified with carbon nanotubes (SPE/CNT). To study the effectiveness of electroenzymatic reactions catalyzed by cytochrome P450 2C9, the nonsteroidal anti-inflammatory drug diclofenac was used as a substrate. Cytochrome P450 2C9 catalyzes the stereospecific hydroxylation reaction to form 4'-hydroxydiclofenac. The metabolite 4'-hydroxydiclofenac was recorded at a potential E = + 0.12 (relative to Ag/AgCl).The use of FAD and FMN as low-molecular mediators made it possible to increase the efficiency of electrocatalysis of the SPE/DDAB/CYP2С9/FAD system to 148±10% and SPE/DDAB/CYP2С9/FMN to 113±6% compared to SPE/DDAB/CYP2С9 (100±5%), and also increase the rate of the enzymatic reaction by 1.5 and 1.13 times, respectively.

FUNDING

The work was performed within the framework of the Program for Basic Research in the Russian Federation for a long-term period (2021-2030) (No. 122030100168-2).

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