Biomedical Chemistry: Research and Methods, 2018, 1(3), e00039
The 40th Anniversary of the Institute of Physiologically Active Compounds of the Russian Academy of Sciences

Analysis of Immunochemical Parameters in Vertebrogenic Pain Syndrome

A.I. Levashova*, M.A. Myagkova

Institute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severny proezd, Moscow region, Chernogolovka, 142432 Russia,*e-mail:

Key words: vertebrogenic pain syndrome; natural antibodies; β-endorphin; orphanin FQ; serotonin; dopamine

DOI: 10.18097/BMCRM00039

The whole version of this paper is available in Russian.

The immune system can react to pain by synthesis of the natural antibodies to pain-regulating mediators which affect the nervous system. In this study, the analysis of content antibodies to opioid peptides (β-endorphin and orphanin FQ) and biogenic amines (serotonin and dopamine) in vertebrogenic pain syndrome (PS) was carries out in serum of PS patients. It has been found that this PS is accompanied by an increase in the production of antibodies to the neurotransmitters studied, and the immune response to opioid peptides is more profound than to biogenic amines. The antibodies levels were higher in female patients than in males (especially for orphanin FQ). These data indirectly correlate with the PS intensity and the dynamics of its regression, evaluated using a visual analogue scale (VAS). Thus, the pain intensity measured by VAS in women was higher, in compare with men and the dynamics of decrease was lower. In addition, it was found that during the significant decrease of the pain intensity, there was no significant decrease in antibody levels to antinociceptive mediators. Apparently, this observation indicates the antibodies to antinociceptive mediators can effect the mechanisms of transition from acute to chronic pain and in maintenance of chronic pain.

Figure 1. The dynamics of pain decrease in different functional stages (spontaneous pain and pain at activity) in the male and female groups.

Figure 2. The dynamics of pain decrease in different functional stages (night pain and pain at rest) in the male and female groups.

Figure 3. The distribution of patients by the n-Abs levels in the male and female groups.

Table 1. Parameters of the levels of studied natural antibodies (OD450 in ELISA (М±SD)) in serum blood samples of examined individuals (patients and control) in the male and female groups

Table 2. The distribution of patients in the male and female groups depending on the levels of n-Abs under studied by groups: lowered, elevated and high level (%)


The work was carried out within the framework of the Program of Fundamental Scientific Research of the State Academies of Sciences for 2013-2020 (State Registration No. 01200952666). The authors thanks the doctor of the Department of Neurology of RMAPE Moseikin Ilya Aleksandrovich for collecting and providing the clinical and biological material of the patients being examined.


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