Biomedical Chemistry: Research and Methods, 2018, 1(3), e00047
The 40th Anniversary of the Institute of Physiologically Active Compounds of the Russian Academy of Sciences

Biological Activity of Alantolactones in Experiments on Cells

S.G. Klochkov*, S.A. Pukhov, M.E. Neganova, E.S. Dubrovskaya, L.A. Anikina, S.V. Afanasyeva, A.V. Semakov

Institute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severny proezd, Moscow region, Chernogolovka, 142432 Russia;*e-mail:

Key words: alantolactones; cytotoxicity; cancer cell lines

DOI: 10.18097/BMCRM00047

The whole version of this paper is available in Russian.

The results of in vitro studies demonstrate cytotoxic activity alantolactone and isoalantolactone, presented in the elecampane plant (Inula helenium) of the natural sesquiterpene lactones. These compounds show toxicity against human cancer cell lines: MS, A549, HCT116, MCF7, RD and K562. The involvement of the p53 signaling pathway in the death of tumor cells, as well as the contribution of reactive oxygen species (ROS) formation during cell death, was studied.

Figure 1. Structures of isomeric allantolactones.

Figure 2. Dose-dependent curves for alantolactone L1 when exposed to the tumor cell lines obtained as a result of the MTT test (48 hours exposure, n = 3, error bars mean ± SD of the value).

Figure 3. Dose-dependent curves for isoalantolactone L2 when exposed to the tumor cell lines obtained as a result of the MTT test (48 hours exposure, n = 3, error bars mean ± SD of the value).

Table 1. Cytotoxicity of allantolactones (MTT test).

Table 2. Fractal descriptors (Dtot, Dval, Dvdw, Dunb and standard errors Δ) of amino acids (β-sheet).

Table 3. Survival of MCF7, MS and HCT116 cells under the action of lactone L1 and L2 (100 μM) in the presence and in the presence of N-acetylcysteine (NAC) evaluated by the MTT test (48 hours exposure, n = 3)


The equipment of Сentre of collective usage was used when carrying out study. The work is carried out within the framework of the Government task 0090-2017-0018.


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