Biomedical Chemistry: Research and Methods, 2018, 1(3), e00048
The 40th Anniversary of the Institute of Physiologically Active Compounds of the Russian Academy of Sciences

Development of 3D Cell Culture on Ultra-High Molecular Weight Polyethylene (UHMWPE) as the Basis of Cellular Matrix

A.A. Ustyugov1*, M.M. Chicheva1, E.A. Lysikova 1, E.A. Vikhareva1, N.A. Sipyagina1, A.N. Malkova1, E.A. Straumal1, E.V. Bovina1, F.S. Senatov2, A.I. Salimon2, A.V. Maksimkin 2, S.A. Lermontov1

1Institute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severny proezd, Moscow region, Chernogolovka, 142432 Russia,*e-mail:
2National University of Science and Technology, 4 Leninskiy prospekt, Moscow, 119049 Russia

Key words: 3D cell culture modeling; polymers; ultra-high-molecular-weight polyethylene

DOI: 10.18097/BMCRM00048

The whole version of this paper is available in Russian.

The study is devoted to the development of an artificial material based on the ultrahigh-molecular weight polyethylene (UHMWPE) with a porous or cellular 3D structure as a cellular matrix – a framework for growing cell cultures. The development of such matrix provides support for neuronal cell culture under conditions that mimick those that exist in the living body. Typically, in vitro cellular studies are conducted in a 2D format, which limits intercellular interactions, morphology, differentiation, survival, signaling responses, gene expression and proliferation that are found in vivo. Here, we propose to use UHMWPE as a material of the cellular matrix, the ultra-high molecular weight polyethylene. UHMWP is a bioinert substance, wich allows forming a system of open connected pores needed to provide cellular life conditions with supply of nutrients and oxygen as well as the removal of waste products, the possibility of intercellular communication, etc. As a result, the use of UHMWPE as a cellular matrix will allow to study the processes occurring in cells in the 3D environment.

Figure 1. Comparison between 2D cell culture and 3D cell culture showing the main differences between cells behavior and constraints when cultivated in 2D environment, plated on a slide compared to cells cultivated in a 3D matrix.


Studies of the properties of polymers were carried out with the financial support of the Russian Science Foundation (Project No. 18-13-00145). The research of cellular models is carried out within the framework of the State Assignment of the Institute of Physiologically Active Compounds of the Russian Academy of Sciences (No. 0090-2017-0019) and the Program of the Russian Academy of Sciences (Theme No. 48.8). The study was conducted using equipment of Center for Preclinical Trials and Center for collective use of IPAC RAS.


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