Biomedical Chemistry: Research and Methods, 2018, 1(3), e00051
The 40th Anniversary of the Institute of Physiologically Active Compounds of the Russian Academy of Sciences

Protective Effects of the Natural Sesquiterpene Lactones Against Damage Induced by Glutamate
and Peroxide in SK-N-MC Cells

E.S. Dubrovskaya1*, A.V. Semakov1, S.V. Afanasyeva1, M.E. Neganova1, V.I. Kozlovski2, S.G. Klochkov1

1Institute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severny proezd, Moscow region, Chernogolovka, 142432 Russia;*e-mail:
2Branch of Talrose Institute of Energetic Problems of Chemical Physics Russian Academy of Sciences, 1-10 Acad. Semenov prosp., Chernogolovka, Moscow region, 142432 Russia

Key words: sesquiterpene lactones; neuroblastoma; peroxide; glutamate; resazurin

DOI: 10.18097/BMCRM00042

The whole version of this paper is available in Russian.

In the present study we evaluated the effect of natural sesquiterpene lactones of plants of the genus Inula on the cells of neuroblastoma SK-N-MC under conditions of toxic stress induced by H2O2 and glutamate. Lactones increased cell survival under these conditions and the leader compounds were identified in this series. A putative mechanism for the protective action of lactones on cells under condition toxic stress has been proposed.

Figure 1. Structures of sesquiterpene lactones.

Figure 2. Effect of lactones in concentrations of 10 μM (a) and 1 μM (b) on the survival of SK-N-MC cells. Data are expressed as % of control as mean ± SD (n = 3). The control contains only 0.3% DMSO.

Figure 3. The protective action of lactones at a concentration of 1 μM with respect to the toxic effect of glutamate 25 mM (a) and H2O2 60 μM (b) on human SK-N-MC human neuroblastoma cells. (a) Cells were incubated with lactones for 24 hours and then 25 mM glutamate was added for a further 24 hours. (b) Cells were incubated with lactones for 24 hours, followed by addition of 60 μM H2O2 for an additional 24 hours. Data are expressed as% of control (control of 0.3% DMSO) as mean ± SD (n = 3).


The equipment of the Center for Collective Use of the Institute of Physiologically Active Compounds of the Russian Academy of Sciences was used. The work was carried out within the framework of the State Proposal 0090-2017-0018.


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