Measurement of Breast Tissue Estrogens by Liquid Chromatography-Tandem Mass Spectrometry

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Published: Jun 29, 2021
DOI: https://doi.org/10.18097/BMCRM00147
Keywords:
estrogen; breast cancer; breast tissue; LC-MS/MS

Main Article Content

D.A. Olisov
Skolkovo Institute of Science and Technology, Skolkovo, Russian Federation
V.V Chagovets
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, 4 bld. 2 Oparina str., Moscow, 117513 Russia
N.L. Starodubtseva
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, 4 bld. 2 Oparina str., Moscow, 117513 Russia
A.A. Smetnik
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, 4 bld. 2 Oparina str., Moscow, 117513 Russia
V.V. Rodionov
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, 4 bld. 2 Oparina str., Moscow, 117513 Russia
V.V. Kometova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, 4 bld. 2 Oparina str., Moscow, 117513 Russia
K.S. Chingin
East China University of Technology, Nanchang, China
V.E. Frankevich
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, 4 bld. 2 Oparina str., Moscow, 117513 Russia

Abstract

Although estrogen contribution estrogen to breast cancer development is not fully understood, an effective method of their measurement, in the mammary gland might provide additional insight. In this study, we have developed a LC-MS/MS method of simultaneous quantification of estrone and estradiol in breast tissue samples. Analytes were extracted with methyl tert-butyl ether by sonication and derivatized with dansyl chloride. Estrogens were analyzed by liquid chromatography-tandem mass spectrometry with an electrospray ionization source. Accuracy and precision were better than 20% for most concentrations. Although estrone and estradiol levels in normal and malignant breast tissue samples analyzed using our method insignificantly differed. The method developed may be used in further studies aimed at evaluating a role estrogens in breast cancer risk.

Article Details

How to Cite
Olisov, D., Chagovets, V., Starodubtseva, N., Smetnik, A., Rodionov, V., Kometova, V., Chingin, K., & Frankevich, V. (2021). Measurement of Breast Tissue Estrogens by Liquid Chromatography-Tandem Mass Spectrometry. Biomedical Chemistry: Research and Methods, 4(2), e00147. https://doi.org/10.18097/BMCRM00147
Issue
Vol. 4 No. 2 (2021)
Section
EXPERIMENTAL RESEARCH

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