New Highly Sensitive Methods for Electroanalysis of the Catalytic Activity of Enzymes of Medical Significance

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Published: Oct 1, 2024
DOI: https://doi.org/10.18097/BMCRM00225
Keywords:
electrochemical analysis; enzymatic catalysis; asparaginase; inhibitors; substrates; cytochrome P450

Main Article Content

V.V. Shumyantseva
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
L.E. Agafonova
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
T.V. Bulko
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
P.I. Koroleva
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
A.V. Kuzikov
Department of Biochemistry, Pirogov Russian National Research Medical University, 1 Ostrovitianova str., Moscow, 117997 Russia
R.A. Masamrekh
Department of Biochemistry, Pirogov Russian National Research Medical University, 1 Ostrovitianova str., Moscow, 117997 Russia
T.A. Filippova
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia

Abstract

The review is devoted to new highly effective methods for analyzing the catalytic activity of enzymes of medical significance, such as cytochromes P450, trypsin, asparaginase, beta-lactamase, and nucleases. The methods are based on registration the specific activity of enzymes using electroanalytical methods. The review analyzes the experimental data obtained by the authors. Two platforms have been developed that allow quantitative measurement of catalytic activity based on the electrochemical properties of the enzyme (cytochrome P450, bactosomes, asparaginase) or substrate (trypsin, nucleases, restriction enzymes, beta-lactamase).

Article Details

How to Cite
Shumyantseva, V., Agafonova, L., Bulko, T., Koroleva, P., Kuzikov, A., Masamrekh, R., & Filippova, T. (2024). New Highly Sensitive Methods for Electroanalysis of the Catalytic Activity of Enzymes of Medical Significance. Biomedical Chemistry: Research and Methods, 7(3), e00225. https://doi.org/10.18097/BMCRM00225
Issue
Vol. 7 No. 3 (2024)
Section
REVIEWS

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