Cytochromes P450 as Tools for Electroenzymatic Synthesis
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Abstract
The electrocatalytic properties of cytochrome P450 2C9 and the cytochrome P450 2C9/FAD and cytochrome P450 2C9/FMN complexes have been studied using a two-electrode system. The system consisted of an enzymatic catalyst electrode modified by the membrane-like compound didodecyldimethylammonium bromide (SPE/DDAB) and a measuring electrode, modified with carbon nanotubes (SPE/CNT). To study the effectiveness of electroenzymatic reactions catalyzed by cytochrome P450 2C9, the nonsteroidal anti-inflammatory drug diclofenac was used as a substrate. Cytochrome P450 2C9 catalyzes the stereospecific hydroxylation reaction to form 4′-hydroxydiclofenac. The metabolite 4′-hydroxydiclofenac was recorded at a potential E=+0.12 (relative to Ag/AgCl).The use of FAD and FMN as low-molecular mediators made it possible to increase the efficiency of electrocatalysis of the SPE/DDAB/CYP2C9/FAD system to 148±10% and SPE/DDAB/CYP2C9/FMN to 113±6% compared to SPE/DDAB/CYP2C9 (100±5%), and also increase the rate of the enzymatic reaction by 1.5 and 1.13 times, respectively.
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References
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