Biomedical Chemistry: Research and Methods 2022, 5(3), e00178

Algorithms for Calculation of Parameters of Electrochemical Biosensor

V.V. Shumyantseva1,2*, V.V. Pronina1

1Institute of Biomedical Chemistry, Pogodinskaya Street, 10, Moscow 119121, Russia; *e-mail:

2Pirogov Russian National Research Medical University, Moscow, Russia

Keywords: electrochemical biosensor; electroactive electrode surface; binding constant; DNA analysis; pharmacogenomics


The whole version of this paper is available in Russian.

The aim of this work is to present the experimental results in the form of an algorithm for analyzing the modification of screen printed electrodes, including the possibility of its regeneration for irreversibly oxidizing biologically active compounds (drugs, DNA and proteins). A protocol was developed for quantitative analysis and study of the mechanism of drug-DNA interaction by differential pulse voltammetry, including the following parameters: complex binding constant, Gibbs free energy, and electrochemical coefficients of the toxic effect.

Figure 1. (A) Distribution diagram of functionalized carbon nanotubes by the size of their outer diameter. (B) Distribution diagram of functionalized carbon nanotubes by the size of their inner diameter. Horizontal lines represent the distribution of diameter of carbon nanotubes.
Figure 2. (A) Cyclic voltammograms of SPE modified successively with dispersions of fCNT and TiO2. The measurements were carried out with different potential sweep rates in 5 mM potassium hexacyanoferrate solution. (B) Plot of IRed against v1/2 for modified SPE/fCNT/TiO2. (SPE – screen-printed electrode, fCNT – functionalized carbon nanotubes).
Figure 3. (А) DVPs SPE/fCNT/TiO2 of various dsDNA concentrations. 100 mM potassium phosphate buffer with 50 mM NaCl as supporting electrolyte, pH 7.4. Accumulation potential of 0.4 V, time accumulation of 15 min, pulse amplitude of 0.025 V, step capacity of 0.005 V, interval time of 50 ms, modulation amplitude of 0.05 V. All potentials were referred to the Ag/AgCl reference electrode. (B) Calibration curve of peak currents against dsDNA concentrationfor guanine. (C) Calibration curve of peak currents against dsDNA concentration for adenine. (SPE – screen-printed electrode, fCNT – functionalized carbon nanotubes).
Figure 4. (А) DVPs SPE/fCNT/dsDNA and SPE/fCNT/dsDNA/Abiraterone acetate of various Abiraterone acetate concentrations. Pulse amplitude of 0.025 V, step capacity of 0.005 V, interval time of 50 ms, modulation amplitude of 0.05V. (SPE – screen-printed electrode, fCNT – functionalized carbon nanotubes).
Figure 5. DVPs SPE/fCNT/TiO2 with dsDNA concentration of 3 mg/ml. (SPE – screen-printed electrode, fCNT – functionalized carbon nanotubes).

Table 1. Electroanalytical characteristics of screen printed electrodes (SPE) modified with fCNT/TiO2 obtained in a 5 mM solution of potassium hexacyanoferrate. The potential values and peaks of oxidation and reduction of potassium ferricyanide are presented for a potential sweep rate of 50 mV/s. (fCNT – functionalized carbon nanotubes).

Table 2. Electroactive areas of SPE modified with various dispersions ((SPE – screen-printed electrode, fCNT – functionalized carbon nanotubes).

Table 3. Metrological and electrochemical parameters of the electrodes.


The work was performed within the framework of the Program for Basic Research in the Russian Federation for a long-term period (2021-2030) (№122030100168-2).


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