Measurement of Breast Tissue Estrogens by Liquid Chromatography-tandem Mass Spectrometry

Authors

  • D.A. Olisov Skolkovo Institute of Science and Technology, Skolkovo, Russian Federation
  • V.V Chagovets Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, 4 bld. 2 Oparina str., Moscow, 117513 Russia
  • N.L. Starodubtseva Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, 4 bld. 2 Oparina str., Moscow, 117513 Russia
  • A.A. Smetnik Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, 4 bld. 2 Oparina str., Moscow, 117513 Russia
  • V.V. Rodionov Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, 4 bld. 2 Oparina str., Moscow, 117513 Russia
  • V.V. Kometova Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, 4 bld. 2 Oparina str., Moscow, 117513 Russia
  • K.S. Chingin East China University of Technology, Nanchang, China
  • V.E. Frankevich Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, 4 bld. 2 Oparina str., Moscow, 117513 Russia

DOI:

https://doi.org/10.18097/BMCRM00147

Keywords:

estrogen; breast cancer; breast tissue; LC-MS/MS

Abstract

Although estrogen contribution estrogen to breast cancer development is not fully understood, an effective method of their measurement, in the mammary gland might provide additional insight. In this study, we have developed a LC-MS/MS method of simultaneous quantification of estrone and estradiol in breast tissue samples. Analytes were extracted with methyl tert-butyl ether by sonication and derivatized with dansyl chloride. Estrogens were analyzed by liquid chromatography-tandem mass spectrometry with an electrospray ionization source. Accuracy and precision were better than 20% for most concentrations. Although estrone and estradiol levels in normal and malignant breast tissue samples analyzed using our method insignificantly differed. The method developed may be used in further studies aimed at evaluating a role estrogens in breast cancer risk.

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Published

2021-06-29

How to Cite

Olisov, D., Chagovets, V., Starodubtseva, N., Smetnik, A., Rodionov, V., Kometova, V., Chingin, K., & Frankevich, V. (2021). Measurement of Breast Tissue Estrogens by Liquid Chromatography-tandem Mass Spectrometry. Biomedical Chemistry: Research and Methods, 4(2), e00147. https://doi.org/10.18097/BMCRM00147

Issue

Section

EXPERIMENTAL RESEARCH