Properties of Calcium-Activated Chloride Currents in Rat Purkinje Cerebellum Neurons

  • V.L. Zamoyski Institute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severny proezd, Moscow region, Chernogolovka, 142432 Russia
  • E.V. Bovina Institute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severny proezd, Moscow region, Chernogolovka, 142432 Russia
  • V.V. Grigoriev Institute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severny proezd, Moscow region, Chernogolovka, 142432 Russia
Keywords: patch-clamp method, Purkinje cerebellum cells, calcium-activated chloride current, tetraethylammonium, 4-aminopyridin, nifuminic acid

Abstract

The presence of calcium-activated chloride current was shown using on freshly isolated rat Purkinje cerebellum neiurones and the pacth-clamp method in the whole-cell configuration. Chloride currents appeared in sodium-free external solution and reversibly disappeared in chloride-free or calcium-free external solution. Replacing of K+ ions (120 mM) to Cs+ ions in micropipette (120 mM) show the chloride currents even with 140 mM Na+ in external solution. This current was blocked to 80–100% by nifluminic acid (25–100 μM). It was found out that well known blockers of potassium channels tetraethylammonium (TEA) and 4-aminopyridine (4-AP) also effectively blocked chloride channels. The IC50 values for TEA and 4-AP were 130 μM, and 110 μM respectively. The action of TEA was reversible, while 4-AP at concentration 100 μM and above irreversibly blocked chloride channels.

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Published
2018-10-01
Section
Experimental Research