Application of the SPR Biosensor in Drug Prototypes Discovery with Human Cytochrome P450(51) as an Example

  • L.A. Kaluzhskiy Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
  • P.V. Ershov Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
  • T.V. Shkel Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, 5/2 Acad. Kuprevich str., Minsk, 220141 Belarus
  • O.V. Gnedenko Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
  • N.V. Ivanchina Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, 159, 100 Let Vladivostoka ave., Vladivostok, 690022 Russia
  • N.V. Strushkevich Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, 5/2 Acad. Kuprevich str., Minsk, 220141 Belarus
  • A.A. Kicha Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, 159, 100 Let Vladivostoka ave., Vladivostok, 690022 Russia
  • I.P. Grabovec Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, 5/2 Acad. Kuprevich str., Minsk, 220141 Belarus
  • A.A. Gilep Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, 5/2 Acad. Kuprevich str., Minsk, 220141 Belarus
  • S.A. Usanov Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, 5/2 Acad. Kuprevich str., Minsk, 220141 Belarus
  • V.A. Stonik Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, 159, 100 Let Vladivostoka ave., Vladivostok, 690022 Russia
  • A.S. Ivanov Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
Keywords: surface plasmon resonance (SPR); screening; human cytochrome P450(51); drug prototypes

Abstract

The development of the integral platform “From Gene to Lead”, consolidated computer methods, bioinformatics researches, and experimental approaches, significantly accelerated and optimized base structure search in the field of drug design. The necessity of the experimental verification of hundreds virtual structure hypothesis (results of molecular data base selections or de novo construction) requires demands the usage of the high-through out and sensitive methods for validation possible interaction between numerous of selected compounds and particular molecular targets and evaluation of affinity, kinetics and thermodynamics. Surface plasmon resonance (SPR) technology makes it possible to solve all these problems. In this article the methodical aspects of the optical SPR-biosensor usage in the field of drug prototypes selection are described using the human cytochrome P450(51) catalyzing one of the key step of cholesterol biosynthesis as an example.

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Published
2018-11-14
Section
Protocols of Experiments, Useful Models, Programs and Services