TY - JOUR AU - Shapovalova, V.V. AU - Radko, S.P. AU - Ptitsyn, K.G. AU - Krasnov, G.S. AU - Nakhod, K.V. AU - Konash, O.S. AU - Vinogradina, M.A. AU - Ponomarenko, E.A. AU - Druzhilovskiy, D.S. AU - Lisitsa, A.V. PY - 2020/12/25 Y2 - 2024/03/29 TI - Processing Oxford Nanopore Long Reads Using Amazon Web Services JF - Biomedical Chemistry: Research and Methods JA - BIOMED CHEM RM VL - 3 IS - 4 SE - PROTOCOLS OF EXPERIMENTS, USEFUL MODELS, PROGRAMS AND SERVICES DO - 10.18097/BMCRM00131 UR - http://bmc-rm.org/index.php/BMCRM/article/view/131 SP - e00131 AB - <p>Studies of genomes and transcriptomes are performed using sequencers that read the sequence of nucleotide residues of genomic DNA, RNA, or complementary DNA (cDNA). The analysis consists of an experimental part (obtaining primary data) and bioinformatic processing of primary data. The bioinformatics part is performed with different sets of input parameters. The selection of the optimal values of the parameters, as a rule, requires significant computing power. The article describes a protocol for processing transcriptome data by virtual computers provided by the cloud platform Amazon Web Services (AWS) using the example of the recently emerging technology of long DNA and RNA sequences (Oxford Nanopore Technology). As a result, a virtual machine and instructions for its use have been developed, thus allowing a wide range of molecular biologists to independently process the results obtained using the "Oxford nanopore".</p> ER -