Construction of a Chimeric Human Gene Encoding Renalase with a Modified N-terminus

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Published: Sep 30, 2020
DOI: https://doi.org/10.18097/BMCRM00137
Keywords:
signal sequence; renalase; expression; cloning; PCR

Main Article Content

V.I. Fedchenko
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
A.A. Kaloshin
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
N.I. Kozlova
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
A.T. Kopylov
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
A.E. Medvedev
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia

Abstract

Renalase (RNLS) is a recently discovered protein that plays different roles inside and outside cells. Extracellular RNLS exhibits protective effects on the cell, acting on its receptor proteins, while intracellular RNLS acts as FAD-dependent oxidoreductase (EC 1.6.3.5). The ratio of the intracellular and extracellular forms of this protein, as well as the mechanisms and factors responsible for its transport from the cell, remain unknown. One of the approaches to studying these issues can be the creation of chimeric forms of this protein with modified fragments of its amino acid sequences. This work describes a method for constructing a chimeric human RNLS gene encoding RNLS without its N-terminal peptid

Article Details

How to Cite
Fedchenko, V., Kaloshin, A., Kozlova, N., Kopylov, A., & Medvedev, A. (2020). Construction of a Chimeric Human Gene Encoding Renalase with a Modified N-terminus. Biomedical Chemistry: Research and Methods, 3(3), e00137. https://doi.org/10.18097/BMCRM00137
Issue
Vol. 3 No. 3 (2020)
Section
EXPERIMENTAL RESEARCH

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