SPR Biosensor Based High-Throughput Screening of Low Molecular Weight Compounds for Interaction with Candida krusei CYP51

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Published: Mar 30, 2023
DOI: https://doi.org/10.18097/BMCRM00183
Keywords:
surface plasmon resonance; screening; cytochrome P450 51; low weight compounds

Main Article Content

L.A. Kaluzhskiy
Institute of Biomedical Chemistry, 10/8 Pogodinskaya str., Moscow, 119121 Russia
T.V. Tsybruk
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, 5/2 Acad. Kuprevich Street, Minsk, 220141 Belarus
E.O. Yablokov
Institute of Biomedical Chemistry, 10/8 Pogodinskaya str., Moscow, 119121 Russia
O.V. Gnedenko
Institute of Biomedical Chemistry, 10/8 Pogodinskaya str., Moscow, 119121 Russia
E.A. Zelepuga
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Science, 159 Prospect 100-letiya Vladivostoka, Vladivostok, 690022 Russia
A.A. Kicha
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Science, 159 Prospect 100-letiya Vladivostoka, Vladivostok, 690022 Russia
E.P. Kozlovskaya
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Science, 159 Prospect 100-letiya Vladivostoka, Vladivostok, 690022 Russia
N.V. Ivanchina
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Science, 159 Prospect 100-letiya Vladivostoka, Vladivostok, 690022 Russia
A.A. Gilep
Institute of Biomedical Chemistry, 10/8 Pogodinskaya str., Moscow, 119121 Russia; Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, 5/2 Acad. Kuprevich Street, Minsk, 220141 Belarus
A.S. Ivanov
Institute of Biomedical Chemistry, 10/8 Pogodinskaya str., Moscow, 119121 Russia

Abstract

The opportunistic fungus Candida krusei is the causative agent of nosocomial infections characterized by high mortality and development of resistance to drugs of the azole class. Therefore, develjoment of non-azole antifungal agents against resistant fungal strains is extremly important. Lanosterol 14-alpha demethylase (CYP51) is a well-known antifungal target. The optical SPR biosensor is a universal tool for screening studies in search of new drug prototypes. This paper presents the methodological aspects of high-hroughput SPR based screening of a library of low molecular weight compounds of natural origin for their interaction with C. krusei CYP51. It has been shown that when performing high-throughput screening, a researcher should pay special attention to the degree of a sensorgram curvature in the association phase. The described approaches to the analysis of high throughput screening data can be useful for researchers working with SPR biosensors from various manufacturers.

Article Details

How to Cite
Kaluzhskiy, L., Tsybruk, T., Yablokov, E., Gnedenko, O., Zelepuga, E., Kicha, A., Kozlovskaya, E., Ivanchina, N., Gilep, A., & Ivanov, A. (2023). SPR Biosensor Based High-Throughput Screening of Low Molecular Weight Compounds for Interaction with Candida krusei CYP51. Biomedical Chemistry: Research and Methods, 6(1), e00183. https://doi.org/10.18097/BMCRM00183
Issue
Vol. 6 No. 1 (2023)
Section
PROTOCOLS OF EXPERIMENTS, USEFUL MODELS, PROGRAMS AND SERVICES

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