Interaction of Mouse and Sheep Polyclonal Antibodies with the Main Forms of Human and Rat Renalase

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Published: Oct 28, 2024
DOI: https://doi.org/10.18097/BMCRM00248
Keywords:
polyclonal antibodies; human renalase-1 (RNLS1-human); rat renalase-2 (RNLS2-rat)

Main Article Content

V.I. Fedchenko
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
A.A. Kaloshin
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
S.A. Kaloshina
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
A.E. Medvedev
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia

Abstract

The interaction of sheep and mouse polyclonal antirenalase antibodies obtained by immunization with full-length human (RNLS1-human) and rat (RNLS2-rat) renalases, respectively, has been studied. The target recombinant proteins, RNLS1-human and RNLS2-rat, were expressed in E. coli cells and isolated by Ni-agarose chromatography. Sheep polyclonal antibodies against RNLS1-human interacted more effectively with both RNLS1-human than with RNLS2-rat. Mouse polyclonal antibodies against RNLS2-rat effectively interacted mainly with RNLS2-rat, but not with RNLS1-human. The data obtained indicate the preferential selectivity of the antibody interaction with the proteins against which they were obtained. This should be taken into consideration in the case of selection of commercially available antibody preparations for quantitative immunodetection of target proteins in biological objects.

Article Details

How to Cite
Fedchenko, V., Kaloshin, A., Kaloshina, S., & Medvedev, A. (2024). Interaction of Mouse and Sheep Polyclonal Antibodies with the Main Forms of Human and Rat Renalase. Biomedical Chemistry: Research and Methods, 7(4), e00248. https://doi.org/10.18097/BMCRM00248
Issue
Vol. 7 No. 4 (2024): Online first
Section
EXPERIMENTAL RESEARCH

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