Protocol for the Preparative Isolation of LDL and HDL Fractions from Human Blood Plasma
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Abstract
The pathogenesis of atherosclerosis, the main cause of cardiovascular disease, including coronary heart disease and stroke, involves plasma lipoproteins, which differ in protein and lipid composition, size, charge and function. At present, chromatographic methods, in particular gel filtration (SEC), are widely used to isolate lipoproteins. In this article, we present a protocol for the preparative isolation of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) fractions from human blood plasma using gel chromatography. To determine the boundaries of the elution zones of apolipoproteins A-1 and B-100, HDL and LDL markers, respectively, we used liquid chromatography with tandem mass spectrometry (LC-MS/ MS) with ELISA cross-validation. Lipoprotein fractions obtained with the help of the SEC can be used for various studies of scientific and applied importance.
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References
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