Determination of Cholesterol and Triglyceride Concentrations in Serum Extracellular Vesicles Using Commercial Kits

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Published: Jun 29, 2021
DOI: https://doi.org/10.18097/BMCRM00148
Keywords:
triglycerides; cholesterol; dynamic light scattering; blood serum; small extracellular vesicles

Main Article Content

A.A. Yakovlev
Institute of Higher Nervous Activity and Neurophysiology RAS, 5A Butlerova str., Moscow, 117485 Russia; Scientific and Practical Psychoneurological Center named after Z.P. Solovy’ov DZM, 43 Donskaya str., Moscow, 115419 Russia
V.D. Antonov
Moscow Institute of Physics and Technology (State University), 9 Institutskiy per., Dolgoprudny, Moscow region, Russia
T.A. Druzhkova
Scientific and Practical Psychoneurological Center named after Z.P. Solovy’ov DZM, 43 Donskaya str., Moscow, 115419 Russia
A.B. Guekht
Scientific and Practical Psychoneurological Center named after Z.P. Solovy’ov DZM, 43 Donskaya str., Moscow, 115419 Russia
N.V. Gulyaeva
Institute of Higher Nervous Activity and Neurophysiology RAS, 5A Butlerova str., Moscow, 117485 Russia; Scientific and Practical Psychoneurological Center named after Z.P. Solovy’ov DZM, 43 Donskaya str., Moscow, 115419 Russia

Abstract

Exosomes and microvesicles, collectively referred to as small extracellular vesicles (sEV) are vesicles with an average size of about 100-150 nm. Currently, the role of sEV in various aspects of signaling in the body is being actively investigated; in addition, sEV can often serve as markers of various pathologies. The active study of the sEV composition is continuing. In this study we have demonstrated that in sEV it is possible to determine cholesterol and triglycerides concentration by using commercial kits designed for serum. The technique was tested on sEV from the blood of patients diagnosed with depression and on healthy volunteers. No differences were found in the concentration of cholesterol and triglycerides in mEV from the blood serum of depressed patients and the control group. The concentration of cholesterol and triglycerides in the samples is several times higher than the sensitivity threshold of the methods set by the manufacturer of the kits.

Article Details

How to Cite
Yakovlev, A., Antonov, V., Druzhkova, T., Guekht, A., & Gulyaeva, N. (2021). Determination of Cholesterol and Triglyceride Concentrations in Serum Extracellular Vesicles Using Commercial Kits. Biomedical Chemistry: Research and Methods, 4(2), e00148. https://doi.org/10.18097/BMCRM00148
Issue
Vol. 4 No. 2 (2021)
Section
EXPERIMENTAL RESEARCH

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