Fluoroquinolone-Induced NAD⁺ Depletion as a Novel Mechanism of Cytotoxicity
Main Article Content
Abstract
A potential mechanism for the cytotoxic and antitumor effects of fluoroquinolones has been discovered. Treatment of HCT-116wt cells with norfloxacin, ciprofloxacin, and ofloxacin decreased intracellular NAD⁺ and cell survival. The IC50 values for the effect of fluoroquinolones were 0.314 mM (for norfloxacin), 0.547 mM (for ciprofloxacin), and 0.612 mM (for ofloxacin). The MTT test for cytotoxicity and mitochondrial functional status assessment revealed similar IC50 values of 0.32 mM (for norfloxacin), 0.35 mM (for ciprofloxacin), and 0.67 mM (for ofloxacin). The suggest unveil a new potential mechanism of fluoroquinolone action on cells beyond their antimicrobial activity.
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How to Cite
Kuzminov, I., Boyarintsev, D., Bryutova, K., Melnik, A., & Rodina, Y. (2026). Fluoroquinolone-Induced NAD⁺ Depletion as a Novel Mechanism of Cytotoxicity. Biomedical Chemistry: Research and Methods, 9(2), e00294. https://doi.org/10.18097/BMCRM00294
Section
EXPERIMENTAL RESEARCH
References
- Baggio, D.,Ananda-Rajah, M. R. (2021) Fluoroquinolone antibiotics and adverse events. Australian Prescriber, 44(5), 161–164. DOI
- Hryhoriv, H., Kovalenko, S. M., Georgiyants, M., Sidorenko, L., Georgiyants, V. (2023) A comprehensive review on chemical synthesis and chemotherapeutic potential of 3-heteroaryl fluoroquinolone hybrids. Antibiotics (Basel, Switzerland), 12,(3), 625. DOI
- Mondal, E. R., Das, S. K., Mukherjee, P. (2004) Comparative evaluation of antiproliferative activity and induction of apoptosis by some fluoroquinolones with a human non-small cell lung cancer cell line in culture. Asian Pacific Journal of Cancer Prevention : APJCP, 5(2), 196–204.
- Abdel-Aal, M. A. A., Abdel-Aziz, S. A., Shaykoon, M. S. A., Abuo-Rahma, G. E. A. (2019) Towards anticancer fluoroquinolones: a review article. Archiv der Pharmazie, 352(7), e1800376. DOI
- Nowakowska, J., Radomska, D., Czarnomysy, R., & Marciniec, K. (2024). Recent Development of Fluoroquinolone Derivatives as Anticancer Agents. Molecules (Basel, Switzerland), 29 (15), 3538. DOI
- Yadav, V., & Talwar, P. (2019). Repositioning of fluoroquinolones from antibiotic to anti-cancer agents: An underestimated truth. Biomedecine & pharmacotherapie, 111, 934–946. DOI
- Ho, F.Y., Tsang, W.P., Kong, S.K., Kwok, T.T. (2006) The critical role of caspases activation in hypoxia/reoxygenation induced apoptosis. Biochemical and Biophysical Research Communications, 345(3), 1131–1137. DOI
- Asmaa E. Kassab (2025) The most recent updates on the anticancer potential of fluoroquinolones: a mini review, Future Medicinal Chemistry, 17(16), 2067- 2078, DOI
- Yoshino, J., Imai, S. (2013) Accurate measurement of nicotinamide adenine dinucleotide (NAD+) with high-performance liquid chromatography. Methods in Molecular Biology (Clifton, N.J.), 10 (7), 203–215. DOI
- Turnidge J. (1999). Pharmacokinetics and pharmacodynamics of fluoroquinolones. Drugs, 58 (2), 29–36. DOI
- Vallet, C. M., Marquez, B., Ngabirano, E., Lemaire, S., Mingeot-Leclercq, M. P., Tulkens, P. M., & Van Bambeke, F. (2011). Cellular accumulation of fluoroquinolones is not predictive of their intracellular activity: studies with gemifloxacin, moxifloxacin and ciprofloxacin in a pharmacokinetic/ pharmacodynamic model of uninfected and infected macrophages. International journal of antimicrobial agents, 38(3), 249–256. DOI