Сравнительный анализ биоэлектрокаталитических систем, содержащих цитохром Р450 3А4

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Опубликован: Mar 15, 2024
DOI: https://doi.org/10.18097/BMCRM00210
Ключевые слова:
электроанализ; лекарственные препараты; цитохром Р450 3А4; биореактор; ферментативный катализ; электроферментативные системы; флавиновые нуклеотиды; бактосомы

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П.И. Королева
Научно-исследовательский институт Биомедицинской химии им. В.Н. Ореховича, 119121 Москва, Погодинская ул., 10
В.В. Шумянцева
Научно-исследовательский институт Биомедицинской химии им. В.Н. Ореховича, 119121 Москва, Погодинская ул., 10

Аннотация

Описаны разработанные авторами подходы для повышения эффективности электроферментативных реакций, катализируемых цитохромом Р450 3А4. Проведен сравнительный анализ цитохром Р450 3А4-систем (i) при образовании функциональных комплексов гемопротеин-флавиновые нуклеотиды как низкомолекулярные модели NADPH-зависимой цитохром Р450 редуктазы, (ii) при образовании продуктивного фермент-субстратного комплекса до стадии получения электронов, поступающих с электрода, (iii) при включении фермента в нанопоры различной природы на электроде (2D-3D переход). Рассмотрены результаты по электрохимическому восстановлению бактосом как функционально активных моделей микросомальной монооксигеназной системы. Для сравнения результатов, полученных для разных моделей, были исследованы электрохимические и электрокаталитические параметры цитохрома Р450 3A4 и маркерного субстрата эритромицина.

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Как цитировать
Королева P., & Шумянцева V. (2024). Сравнительный анализ биоэлектрокаталитических систем, содержащих цитохром Р450 3А4. Biomedical Chemistry: Research and Methods, 7(1), e00210. https://doi.org/10.18097/BMCRM00210
Выпуск
Том 7 № 1 (2024)
Раздел
ПРОТОКОЛЫ ЭКСПЕРИМЕНТОВ, ПОЛЕЗНЫЕ МОДЕЛИ, ПРОГРАММЫ И СЕРВИСЫ

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