Протоколы протеомного анализа: выделение, солюбилизация белков и гидролиз белков протеазами

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Опубликован: Jun 20, 2025
DOI: https://doi.org/10.18097/BMCRM00277
Ключевые слова:
протеомика; солюбилизация белков; высокоспецифичная протеаза; протокол

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Е.Н. Обухова
Сколковский институт науки и технологий, 121205, Москва, Большой бул., 30с1
Е.В. Хряпова
Научно-исследовательский институт биомедицинской химии им. В.Н. Ореховича, 119435, Москва, Погодинская ул., 10
С.Е. Новикова
Научно-исследовательский институт биомедицинской химии им. В.Н. Ореховича, 119435, Москва, Погодинская ул., 10
Л.Ш. Казиева
Научно-исследовательский институт биомедицинской химии им. В.Н. Ореховича, 119435, Москва, Погодинская ул., 10
О.В. Тихонова
Научно-исследовательский институт биомедицинской химии им. В.Н. Ореховича, 119435, Москва, Погодинская ул., 10
В.Г. Згода
Научно-исследовательский институт биомедицинской химии им. В.Н. Ореховича, 119435, Москва, Погодинская ул., 10

Аннотация

Высокопроизводительные исследования белкового состава биологических образцов в настоящее время стали рутинными и используются практически во всех направлениях науки о жизни. Современные методы протеомики позволяют провести достоверную идентификацию и количественную оценку тысяч белков в одном эксперименте. Стандартная процедура протеомного анализа включает следующие стадии: 1. солюбилизация и выделение белков и их последующий гидролиз протеазами; 2. анализ образовавшихся пептидов методом высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектированием; 3. биоинформатическая и статистическая обработка результатов. В данной работе представлены протоколы первого этапа протеомного анализа – пробоподготовки, которые рутинно используются в лаборатории системной биологии Института биомедицинской химии им. В.Н. Ореховича.

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Как цитировать
Обухова E., Хряпова E., Новикова S., Казиева L., Тихонова O., & Згода V. (2025). Протоколы протеомного анализа: выделение, солюбилизация белков и гидролиз белков протеазами. Biomedical Chemistry: Research and Methods, 8(2), e00277. https://doi.org/10.18097/BMCRM00277
Выпуск
Том 8 № 2 (2025)
Раздел
ПРОТОКОЛЫ ЭКСПЕРИМЕНТОВ, ПОЛЕЗНЫЕ МОДЕЛИ, ПРОГРАММЫ И СЕРВИСЫ

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