De novo секвенирование белков и пептидов: алгоритмы, приложения, перспективы

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Опубликован: Apr 12, 2018
DOI: https://doi.org/10.18097/BMCRM00005
Ключевые слова:
протеомика; масс-спектрометрия; de novo секвенирование; белки; пептиды; аминокислотная последовательность

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К.В. Вяткина
Санкт-Петербургский национальный исследовательский Академический университет Российской Академии Наук; 194021, Санкт-Петербург, ул. Хлопина, д. 8, к. 3; Санкт-Петербургский государственный университет; 199034, Санкт-Петербург, Университетская наб., д. 7-9

Аннотация

Установление первичной структуры белков и пептидов является важным этапом при изучении их свойств. В настоящее время для решения данной задачи наиболее часто используется масс-спектрометрия. Результаты масс-спектрометрических измерений могут быть интерпретированы посредством поиска в базе данных или методами de novo секвенирования. Привлекательность последних обусловлена возможностью их применения для исследования неизвестных белков, а также тех, которые не могут быть проанализированы методами геномики или транскриптомики. В данной статье предлагается краткий обзор существующих подходов к de novo секвенированию белков и пептидов и решаемых с их помощью задач, а в завершение обозначены направления и перспективы их дальнейшего развития.

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Как цитировать
Вяткина K. (2018). De novo секвенирование белков и пептидов: алгоритмы, приложения, перспективы. Biomedical Chemistry: Research and Methods, 1(1), e00005. https://doi.org/10.18097/BMCRM00005
Выпуск
Том 1 № 1 (2018)
Раздел
ОБЗОРЫ

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