Перспективы использования секвенаторов третьего поколения для количественного профилирования транскриптома

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Опубликован: Dec 20, 2018
DOI: https://doi.org/10.18097/BMCRM00086
Ключевые слова:
секвенирование третьего поколения; транскриптом; количественное профилирование

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С.П. Радько
Научно-исследовательский институт биомедицинской химии имени В.Н. Ореховича, 119121, Москва, ул. Погодинская, 10
Л.К. Курбатов
Научно-исследовательский институт биомедицинской химии имени В.Н. Ореховича, 119121, Москва, ул. Погодинская, 10
К.Г. Птицын
Научно-исследовательский институт Аджиномото-Генетика, 117545, Москва, 1-й Дорожный проезд, 1
Я.Ю. Киселёва
Российский научный центр рентгенорадиологии, 117997, Москва, ул. Профсоюзная, 86
Е.А. Пономаренко
Научно-исследовательский институт биомедицинской химии имени В.Н. Ореховича, 119121, Москва, ул. Погодинская, 10
А.В. Лисица
Научно-исследовательский институт биомедицинской химии имени В.Н. Ореховича, 119121, Москва, ул. Погодинская, 10
А.И. Арчаков
Научно-исследовательский институт биомедицинской химии имени В.Н. Ореховича, 119121, Москва, ул. Погодинская, 10

Аннотация

Транскриптомное профилирование широко используется для анализа динамики транскриптома при исследовании различных биологических процессов на клеточном и тканевом уровне. В отличие от секвенаторов второго поколения, которые секвенируют относительно короткие фрагменты нуклеиновых кислот, ДНК/РНК секвенаторы третьего поколения, разработанные биотехнологическими компаниями “PacBio” и “Oxford Nanopore Technologies”, позволяют секвенировать транскрипты как единичные молекулы и могут рассматриваться как потенциальные молекулярные счётчики, способные измерять количество копий каждого транскрипта с высокой производительностью, чувствительностью и специфичностью. В данном обзоре рассмотрены особенности технологий одномолекулярного секвенирования, предлагаемых компаниями “PacBio” и “Oxford Nanopore Technologies”, и применение технологий одномолекулярного секвенирования для целей транскриптомного анализа, включая анализ изоформ транкриптов. Также обсуждаются перспективы и ограничения их использования для количественного профилирования транскриптома.

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Как цитировать
Радько S., Курбатов L., Птицын K., Киселёва Y., Пономаренко E., Лисица A., & Арчаков A. (2018). Перспективы использования секвенаторов третьего поколения для количественного профилирования транскриптома. Biomedical Chemistry: Research and Methods, 1(4), e00086. https://doi.org/10.18097/BMCRM00086
Выпуск
Том 1 № 4 (2018)
Раздел
ОБЗОРЫ

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