Biomedical Chemistry: Research and Methods 2024, 7(4), e00246

Comparison of the Results of SPR Analysis of Antigen-Antibody Interactions Performed Using Optical Biosensors Biacore X-100 (“Cytiva”, USA) and MI-S200D (“Inter-Bio”, China)

O.V. Gnedenko*, Y.V. Mezentsev, E.O. Yablokov, L.A. Kaluzhskiy, P.V. Ershov, A.A. Gilep, A.S. Ivanov

Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia; *gnedenko.oksana@gmail.com

Keywords: surface plasmon resonance (SPR); optical biosensors; antigen-antibody interaction; rate constants of assoсiation and dissociation of the complex (kon, koff); equilibrium dissociation constant (Kd)

DOI:10.18097/BMCRM00246

The whole version of this paper is available in Russian.

Limited availability of Western scientific equipment, explains a growing need to switch to using scientific instruments made in China. This is especially true in the case of optical biosensors operating on the surface plasmon resonance (SPR) effect. However, comparability of experimental data obtained using Western and Chinese biosensors has not been investigated yet. In this work we have comparedresults of SPR analysis of the kinetics and affinity of interaction of IgG2a and IgG1 antibodies with protein A. Two biosensos Biacore X-100 (“Cytiva”, USA) and MI-S200D (“Inter-Bio”, China) have been used. It was shown that the values of the association rate constants obtained on both devices for two antibodies were close within one order of magnitude. For IgG1 antibodies, the dissociation rate constants were almost identical. Both devices provide high-quality data that are well described by a simple 1:1 model (Langmuir binding).
Figure 1. Appearance and design features of Biacore X-100 and MI-S200D biosensors.

Figure 2. The structure of Biacore CM5 optical chip. A - a chip storage case (left) and an optical chip (right). B - reverse side.

Figure 3. The structure of IB-CM5 optical chip. A – a chip storage case (left) and an optical chip (right). B - elements of an optical chip - a prism holder (left) and a working part of a chip (right), consisting of an optical prism with gold sputtering, a layer of carboxymethylated dextran and a plastic plate with flow-through microfluidic channels.

Figure 4. Schema of flow optical biosensors operating on the SPR effect [12]. The frames highlight the replaceable parts (optical chips): Biacore CM5 (solid line) and IB-CM5 (dashed line).

Figure 5. Sensorgrams of the interaction of IgG2a with immobilized protein A on the CM5 chip of Biacore X-100 (A) and the IB-CM5 chip of MI-S200D (B) in the concentration range from 10 to 1000 nM: 1 - 10 nM, 2 - 25 nM, 3 - 50 nM, 4 - 100 nM, 5 - 500 nM, and 6 - 1000 nM. Sensorgrams of the interaction of IgG1 with immobilized protein A on the CM5 chip of Biacore X-100 (C) and the IB-CM5 chip of MI-S200D (D) in the concentration range from 10 to 1000 nM: 1 - 10 nM, 2 - 25 nM, 3 - 50 nM, 4 - 100 nM, 5 - 500 nM, and 6 - 1000 nM.

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Table 1. Comparison of parameters of Biacore X-100 and MI-S200D biosensors.

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Table 2 Kinetic parameters (kon and koff) and equilibrium dissociation constant Kd of antibody complexes with immobilized protein A.

FUNDING

The work was carried out within the framework of the Program for Basic Research in the Russian Federation for a long-term period (2021–2030) (No. 122030100168-2).

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