Modeling the Blood-brain Barrier Using Rat Brain Cell Cultures

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Published: Jan 21, 2025
DOI: https://doi.org/10.18097/BMCRM00238
Keywords:
blood-brain barrier, endothelial cells, astrocytes, pericytes, cell co-cultivation

Main Article Content

G.E. Leonov
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
I.V. Vakhrushev
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
V.D. Novikova
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
R.Y. Saryglar
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
K.K. Baskaev
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
A.Y. Lupatov
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
I.V. Kholodenko
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
K.N. Yarygin
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia

Abstract

Permeability of the blood-brain barrier (BBB) represents a significant problem for most promising drugs used for treating brain diseases due to its high selectivity. The neurovascular unit, which includes neurons, interneurons, astrocytes, the basal membrane, smooth muscle cells, pericytes, endothelial cells, and the extracellular matrix, forms an anatomically and functionally cohesive structure that ensures effective regulation of cerebral blood flow. In vitro modeling of the BBB is a relevant and practically significant task for studying the penetration of therapeutic agents into the brain. This study presents a BBB model consisting of endothelial cells, pericytes, and astrocytes, which partially mimics the in vivo layers of the BBB. Despite some limitations, such as incomplete matching of astrocyte location, the model demonstrates high expression of tight junction proteins and optimal TEER values of the endothelial cell monolayer, making it suitable for studying the permeability of the BBB to various substances, including drugs and nanoparticles.

Article Details

How to Cite
Leonov, G., Vakhrushev, I., Novikova, V., Saryglar, R., Baskaev, K., Lupatov, A., Kholodenko, I., & Yarygin, K. (2025). Modeling the Blood-brain Barrier Using Rat Brain Cell Cultures. Biomedical Chemistry: Research and Methods, 7(4), e00238. https://doi.org/10.18097/BMCRM00238
Issue
Vol. 7 No. 4 (2024): Online first
Section
PROTOCOLS OF EXPERIMENTS, USEFUL MODELS, PROGRAMS AND SERVICES

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