Algorithm of Targeted (SRM) Methods Development in Mass-spectrometric Studies

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Published: Apr 12, 2018
DOI: https://doi.org/10.18097/BMCRM00006
Keywords:
selected reaction monitoring; peptides; stable isotope labeling; mass spectrometry

Main Article Content

A.T. Kopylov
Institute of Biomedical Chemistry, Moscow, Russia
A.V. Lisitsa
Institute of Biomedical Chemistry, Moscow, Russia
V.G. Zgoda
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia

Abstract

Determination of protein concentration in biological samples is an important task for biological research, as well as for medicine and routine clinical biochemistry.
The introduction of stable isotope-labeled peptide standards (SIS) made it possible to determine accurately absolute protein concentrations in proteomic studies. The correct choice of SIS and the systematic way to develop a s method for selected reaction monitoring (SRM) are very important steps that are crucial for further identification and measurements of protein concentration. In this paper, we summarize our experience of selecting SIS for measuring the protein concentration by SRM. The results are presented in the form of an algorithm that describes the main stages of the SIS selection and the main points in the development of SRM methods for the targeted protein detection and determination of protein concentrations in biological samples.

Article Details

How to Cite
Kopylov, A., Lisitsa, A., & Zgoda, V. (2018). Algorithm of Targeted (SRM) Methods Development in Mass-spectrometric Studies. Biomedical Chemistry: Research and Methods, 1(1), e00006. https://doi.org/10.18097/BMCRM00006
Issue
Vol. 1 No. 1 (2018)
Section
PROTOCOLS OF EXPERIMENTS, USEFUL MODELS, PROGRAMS AND SERVICES

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