Strategy for Experimental Studies of Target Protein Interactomics

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Published: Oct 1, 2024
DOI: https://doi.org/10.18097/BMCRM00224
Keywords:
platform; surface plasmon resonance; complexation; protein subinteractome; affine selection; protein-protein interactions

Main Article Content

P.V. Ershov
Institute of Biomedical Chemistry, 10/8 Pogodinskaya st., Moscow, 119121 Russia
Y.V. Mezentsev
Institute of Biomedical Chemistry, 10/8 Pogodinskaya st., Moscow, 119121 Russia
E.O. Yablokov
Institute of Biomedical Chemistry, 10/8 Pogodinskaya st., Moscow, 119121 Russia
L.A. Kaluzhskiy
Institute of Biomedical Chemistry, 10/8 Pogodinskaya st., Moscow, 119121 Russia
O.V. Gnedenko
Institute of Biomedical Chemistry, 10/8 Pogodinskaya st., Moscow, 119121 Russia
A.A. Gilep
Institute of Biomedical Chemistry, 10/8 Pogodinskaya st., Moscow, 119121 Russia
A.S. Ivanov
Institute of Biomedical Chemistry, 10/8 Pogodinskaya st., Moscow, 119121 Russia

Abstract

It is known that intermolecular interactions of proteins and peptides play a critical role in life processes. Such interactions can be either directly related to the implementation of various functions or play the role of a regulator. Currently, there is no doubt that the majority of proteins function as part of various molecular complexes, the formation of which occurs due to protein-protein interactions (PPIs), the totality of which can be defined as the “protein interactome”. Protein subinteractome studies are critical for studying the functions and regulatory mechanisms of unknown or poorly annotated proteins, understanding the architecture of intracellular molecular machines, and the design of PPI modulators. Previously, we used combinations of experimental approaches, as well as analytical and preparative methods, to study the subinteractomes of functionally different cellular proteins, which allowed us to identify the protein subinteractomes of several clinically significant human proteins. The purpose of this work was to conceptualize the principles of the experimental platform we developed for studying protein subinteractomes and to describe its features in detail.

Article Details

How to Cite
Ershov, P., Mezentsev, Y., Yablokov, E., Kaluzhskiy, L., Gnedenko, O., Gilep, A., & Ivanov, A. (2024). Strategy for Experimental Studies of Target Protein Interactomics. Biomedical Chemistry: Research and Methods, 7(3), e00224. https://doi.org/10.18097/BMCRM00224
Issue
Vol. 7 No. 3 (2024)
Section
REVIEWS

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