The Sturgeon Ovarian Liquid and the Perch Roe Extract are Able to Enhance the Recovery of the Fibroblasts after their Stress-Induced Premature Senescence

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Published: Jun 26, 2018
DOI: https://doi.org/10.18097/BMCRM00011
Keywords:
fibroblasts; premature senescence; oxidative stress; fish roe; fish ovarian liquid.

Main Article Content

M.V. Mikhailova
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
N.F. Belyaeva
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
N.I. Kozlova
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
K.V. Zolotarev
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
A.N. Mikhailov
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
S.B. Podushka
ChNIORH LLC, 10-83 Slavy prospect, St. Petersburg, 192239 Russia

Abstract

Ovarian liquid and fish roe are valuable sources of biologically active compounds. In order to study mechanisms of aging and also to search for biologically active compounds capable of inhibiting aging, we have modelled premature senescence in human embryonic fibroblasts by incubating of cells with H2O2. Cell senescence was assessed by staining for β-galactosidase (SA-β-Gal) at pH 6.0; cell proliferation was further evaluated by the optical method. The dried ovarian liquid of the Siberian sturgeon and the extract of the perch roe were able to enhance recovery of the cells after induction of their premature senescence caused by oxidative stress. In contrast to the extract from perch roe and the extracts from fish muscle, dried ovarian fluid gave such an effect without the growth of proliferation.

Article Details

How to Cite
Mikhailova, M., Belyaeva, N., Kozlova, N., Zolotarev, K., Mikhailov, A., & Podushka, S. (2018). The Sturgeon Ovarian Liquid and the Perch Roe Extract are Able to Enhance the Recovery of the Fibroblasts after their Stress-Induced Premature Senescence. Biomedical Chemistry: Research and Methods, 1(2), e00011. https://doi.org/10.18097/BMCRM00011
Issue
Vol. 1 No. 2 (2018)
Section
EXPERIMENTAL RESEARCH

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