Способность экстракта икры окуня и лиофилизата овариальной жидкости осетра ускорять восстановление фибробластов после стресс-индуцированного преждевременного старения
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Аннотация
Овариальная жидкость и икра рыб представляют собой ценные источники биологически активных соединений. С целью изучения механизмов старения, а также обнаружения биологически-активных соединений, способных ему препятствовать, производили моделирование данного процесса in vitro, инкубируя культуры эмбриональных фибробластов человека с пероксидом водорода. Индуцированное клеточное старение оценивали путём окрашивания на β-галактозидазу (SA-β-Gal) при рН 6.0, пролиферацию клеток - оптическим методом. Лиофилизат овариальной жидкости осетра сибирского (Acipenser baerii) и экстракт икры окуня обыкновенного (Perca fluviatilis) способны усиливать восстановление клеток после индукции их преждевременного старения, вызванного окислительным стрессом. В отличие от экстракта икры окуня и экстрактов из мышечной ткани рыб, высушенная овариальная жидкость оказывала такой эффект без роста пролиферации.
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